SNAT1 Antibody : Biotin (OASE00249-Biotin)

Catalog No: OASE00249-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: S104-32
• Isotype: IgG1
• Host: Mouse
• Conjugation: Biotin
• Application: IHC, WB, ICC
• Additional Information: Background Info: Detects ~50kDa.
  Scientific Background: The sodium-coupled neutral amino acid transporters (SNAT) of the SLC38 gene family include System A subtypes SNAT1, SNAT2 and SNAT4 and System N subtypes SNAT3 and SNAT5. The SLC38 transporters are essential for the uptake of nutrients, energy production, metabolism, detoxification, and the cycling of neurotransmitters. The SNAT1 protein, also designated ATA1 or NAT2 is encoded by the human gene SLC38A1 which maps to chromosome 12q13.11. SNAT1 is responsible for the transport of glutamine, an intermediate in the synthesis of urea, and may be involved in the generation of glutamate in the retina. SNAT1 protein may be detected in some tissues such as heart, brain and placenta and expression levels are enriched in certain neuronal populations within the CNS. SNAT1 is not present in astrocytes.
  Certificate of Analysis: 1ug/mL was sufficient for detection of SNAT1 in 20ug of lysates from neocortical neurons cultured under amino acid starvation conditions and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 1- 63 of rat SNAT1
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 (WB)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Hatanaka T., et al. ( 2000) Biochim. Biophys. Acta 1467: 1-6.
  2. Wang H., et al. (2000) Biochem. Biophys. Res. Commun. 273: 1175-1179.
  3. Gu S., et al. (2001) J. Biol. Chem. 276: 24137-24144.
  4. Freeman T.L., et al. (2002) Arch. Biochem. Biophys. 400: 215-222.
  5. Palii S.S., et al. (2004) J. Biol. Chem. 279: 3463-3471.
  6. Sidoryk M., et al. (2004) Neuroreport. 15: 575-578.
  Erratum in: 2004. Neuroreport. 15: 533.
  7. Desforges,M., et al. (2006) Am. J. Physiol. Cell.
  Physiol. 290: 305-312.
  

Target Info

• Gene Symbol: Slc38a1
• Gene Full Name: solute carrier family 38, member 1
• Alias Symbols: Ata1, GlnT, Sat1
• NCBI Gene Id: 170567
• Uniprot ID: Q9JM15
• Protein Accession #: NP_620187.1