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Catalog No: OCOA13814
Size:20UG
Price: $179.00
SKU
OCOA13814
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SNAPC2 Protein Lysate (OCOA13814)

Datasheets/ManualsPrintable datasheet for SNAPC2 Protein Lysate (OCOA13814)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionSNAPC2 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolSNAPC2
Gene Full Namesmall nuclear RNA activating complex polypeptide 2
Alias Symbolsproximal sequence element-binding transcription factor subunit delta;PSE-binding factor subunit delta;PTF subunit delta;PTFDELTA;small nuclear RNA activating complex, polypeptide 2, 45kD;small nuclear RNA activating complex, polypeptide 2, 45kDa;Small nuclear RNA-activating complex polypeptide 2;SNAP45;SNAPC 45 kDa subunit;SNAPc subunit 2;snRNA-activating protein complex 45 kDa subunit;snRNA-activating protein complex subunit 2.
NCBI Gene Id6618
Protein NameNM_003083, NP_003074, Q13487
Description of TargetHomo sapiens small nuclear RNA activating complex polypeptide 2 (SNAPC2), transcript variant 1, mRNA, snRNA-activating protein complex subunit 2, snRNA-activating protein complex subunit 2 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q13487
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_003074
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_003083
Molecular Weight35.6 kDa
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