SNAP25 ELISA Kit (Rat) (OKEI00849)

Catalog No: OKEI00849

Size:96 Wells

Rat SNAP25 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Snap25 ELISA Kit (Rat) (OKEI00849) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Snap25 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Snap25 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Snap25
• ELISA Kit Range: 0.156 - 10 ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Snap25. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Snap25 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • LACTB2 Microplate: 96 Wells (12 x 8 Well strips)
  • LACTB2 Lyophilized Standard: 2
  • 100X LACTB2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Biological Fluids
• Sensitivity: < 0.10 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SNAP25
• Alias Symbols: SNAP-25B, SNAP-25a
• NCBI Gene Id: 25012
• Protein Name: Synaptosomal-associated protein 25
• Description of Target: t-SNARE involved in the molecular regulation of neurotransmitter release. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells . May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
• Uniprot ID: P60881
• Protein Accession #: NP_001257504
• Nucleotide Accession #: NM_001270575