SNAP25 ELISA Kit (Chicken) (OKEH08129)

Catalog No: OKEH08129

Size:96T

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology SNAP25 ELISA Kit (Chicken) (OKEH08129) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SNAP25 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SNAP25 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SNAP25 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=2.9% (n = 20)
  Mean Inter-assay CV%: <=10.4% (n = 20)
• ELISA Kit Component:
  • SNAP25 Microplate: 96 Wells (12 x 8 Well strips)
  • SNAP25 Lyophilized Standard: 2
  • 100X Biotinylated SNAP25 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion.
  Subcellular Location: Membrane Lipid-anchor Cell junction Synapse Synaptosome Cell membrane Membrane association requires palmitoylation.
  Unigene: Gga.4807
  SMR: P60878
  String: 9031.ENSGALP00000014635
  KEGG: gga:396444
  Pfam: PF00835
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Chicken Synaptosomal-associated protein 25
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SNAP25
• Gene Full Name: synaptosome associated protein 25
• Alias Symbols: SNAP-25;SUP;super protein;synaptosomal-associated 25 kDa protein;synaptosomal-associated protein 25;synaptosomal-associated protein, 25kDa.
• NCBI Gene Id: 396444
• Protein Name: P60878
• Description of Target: Synaptosomal-associated protein 25
• Uniprot ID: https://www.uniprot.org/uniprot/P60878
• Protein Accession #: NP_990789.1
• Nucleotide Accession #: NM_205458.1