Catalog No: OPCA05204
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OPCA05204
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SNAKE VENOM METALLOPROTEINASE ADAMALYSIN-2 Recombinant Protein (Eastern diamondback rattlesnake) (OPCA05204)

Datasheets/ManualsPrintable datasheet for OPCA05204
Product Info
Predicted Species ReactivityEastern diamondback rattlesnake
Product FormatLiquid or Lyophilized powder
Additional InformationSpecies Specificity Detail: Crotalus adamanteus (Eastern diamondback rattlesnake)
Reconstitution and StorageWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
FormulationTris-base, 50% glycerol
PurityGreater than 90% as determined by SDS-PAGE.
Protein SequenceQQNLPQRYIELVVVADRRVFMKYNSDLNIIRTRVHEIVNIINGFYRSLNIDVSLVNLEIWSGQDPLTIQSSSSNTLNSEGLWREKVLLNKKKKDNAQLLTAIEFKCETLGKAYLNSMCNPRSSVGIVKDHSPINLLVAVTMAHELGHNLGMEHDGKDCLRGASLCIMRPGLTPGRSYEFSDDSMGYYQKFLNQYKPQCILNKP
Storage BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
SourceE. Coli
Protein Range1-203 aa
TagN-terminal 6xHis-tagged
ReferenceFirst structure of a snake venom metalloproteinase: a prototype for matrix metalloproteinases/collagenases. Gomis-Rueth F.-X., Kress L.F., Bode W. EMBO J. 12:4151-4157(1993)
Alias SymbolsAdamalysin II Proteinase II
Protein NameSnake venom metalloproteinase adamalysin-2
Description of TargetHas no significant hemorrhagic activity, but inactivates serpins by limited proteolysis of their reactive-site loops.
Uniprot IDP34179
Protein Size (# AA)1-203 aa
Molecular Weight27.1 kDa
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