SNAI1 ELISA Kit (Human) (OKCD00589)

Catalog No: OKCD00589 (Formerly GWB-KBBBG6)

Size:96WELLS

Human SNAI1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Snail Homolog 1 (SNAI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Snail Homolog 1 (SNAI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Snail Homolog 1 (SNAI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Snail Homolog 1 (SNAI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Snail Homolog 1 (SNAI1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Snail Homolog 1 (SNAI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SNAI1 Microplate: 96 Wells (12 x 8 Well Strips)
  • SNAI1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated SNAI1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.063 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Snail Homolog 1 (SNAI1). No significant cross-reactivity or interference between Snail Homolog 1 (SNAI1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SNAI1
• Gene Full Name: snail family transcriptional repressor 1
• Alias Symbols: dJ710H13.1;protein sna;protein snail homolog 1;SLUGH2;SNA;SNAH;SNAIL;snail 1 homolog;snail 1 zinc finger protein;snail family zinc finger 1;snail homolog 1;SNAIL1;zinc finger protein SNAI1.
• NCBI Gene Id: 6615
• Protein Name: Zinc finger protein SNAI1
• Description of Target: Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (By similarity). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
• Uniprot ID: O95863
• Protein Accession #: NP_005976
• Nucleotide Accession #: NM_005985.3
• Protein Size (# AA): 264