SMPDL3B ELISA Kit (Human) (OKCA01085)

Catalog No: OKCA01085

Size:96 Wells

Human SMPDL3B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	86	83-89
  1:2	92	89-97
  1:4	100	95-105
  1:8	90	87-93

• ELISA Kit Principle: Aviva Systems Biology SMPDL3B ELISA Kit (Human) (OKCA01085) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SMPDL3B has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SMPDL3B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SMPDL3B captured in the well.
• ELISA Kit Range: 0.625 - 40 ng/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	104	100-108
  EDTA Plasma (n=4)	99	95-103

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01085 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01085 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • SMPDL3B Microplate: 96 Wells (12 x 8 Well strips)
  • SMPDL3B Lyophilized Standard: 2 x 40 ng
  • 100X Biotinylated SMPDL3B Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 0.156 ng/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: SMPDL3B
• Gene Full Name: sphingomyelin phosphodiesterase acid like 3B
• Alias Symbols: ASML3B
• NCBI Gene Id: 27293
• Protein Name: Acid sphingomyelinase-like phosphodiesterase 3b
• Description of Target: Lipid-modulating phosphodiesterase (PubMed:26095358). Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling.
• Uniprot ID: Q92485
• Protein Accession #: NP_001009568.1
• Nucleotide Accession #: NM_001009568.2