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Catalog No: OKCA01523
Size:96 Wells
Price: $754.00
SKU
OKCA01523
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat9589-99
1:29791-103
1:49287-98
1:88985-93
ELISA Kit PrincipleAviva Systems Biology SMO ELISA Kit (Human) (OKCA01523) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SMO has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SMO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SMO captured in the well.
ELISA Kit Range23.5 - 1,500 pg/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)10298-105
EDTA Plasma (n=4)8680-91
ELISA Kit Component
ComponentAmount
SMO Microplate96 Wells (12 x 8 Well strips)
SMO Lyophilized Standard2 x 1.5 ng
100X Biotinylated SMO Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity5.8 pg/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolSMO
Gene Full Namesmoothened, frizzled class receptor
Alias SymbolsGx, CRJS, PHLS, SMOH, FZD11
NCBI Gene Id6608
Protein NameSmoothened homolog
Description of TargetG protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.
Uniprot IDQ99835
Protein Accession #NP_005622.1
Nucleotide Accession #NM_005631.4
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