Catalog No: OCOA17443
Size:20UG
Price: $169.00
SKU
OCOA17443
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SMN1 Protein Lysate (OCOA17443)

Datasheets/ManualsPrintable datasheet for SMN1 Protein Lysate (OCOA17443)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionSMN1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolSMN1, SMN2
Gene Full Namesurvival of motor neuron 1, telomeric, survival of motor neuron 2, centromeric
Alias SymbolsBCD541;C-BCD541;component of gems 1;gemin-1;GEMIN1;SMA;SMA@;SMA1;SMA2;SMA3;SMA4;SMN;SMNC;SMNT;survival motor neuron 1 protein;survival motor neuron protein;T-BCD541;TDRD16A;TDRD16B;tudor domain containing 16A;tudor domain containing 16B.
NCBI Gene Id6606, 6607
Protein NameNM_000344, NP_000335, Q16637
Description of TargetHomo sapiens survival of motor neuron 1, telomeric (SMN1), transcript variant d, mRNA, Survival motor neuron protein, survival motor neuron protein isoform d [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q16637
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_000335
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_000344
Molecular Weight31.7 kDa
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