SMC1A Polyclonal Antibody (Human) (ATP00013_T100)

Catalog No: ATP00013_T100

Size:100ul

20 ug of the indicated human whole cell extract was loaded onto a 4-20% SDS-PAGE gel. After electrophoresis, proteins were transferred to a PVDF membrane and probed with 3 ug/mL of antibody. Secondary detection was performed with 0.08 ug/mL of HRP-conjugated donkey anti-rabbit IgG antibody. Images were captured after development of membrane following exposure to AvivaBlot™ Femto ECL Reagent (OKCF00002).

Immunogen: Recombinant protein representing aa 489-667 of Human  SMC1A (green) depicted within in the full length structure prediction (blue)  via AlphaFold. (https://alphafold.ebi.ac.uk/).

Product Info

• Predicted Species Reactivity: Human
• Product Format: PBS w/ 0.09% sodium azide
• Clonality: Polyclonal
• Isotype: IgG
• Application: WB;ICC
• Reconstitution and Storage: Store at -20C
• Immunogen: Recombinant Human SMC1A aa 489-667
• Purification: Protein A
• Concentration: 1 mg/mL

Target Info

• Gene Symbol: Structural maintenance of chromosomes protein 1A
• Gene Full Name: SMC1A
• Alias Symbols: SMC1A
• NCBI Gene Id: 8243
• Description of Target: Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
• Uniprot ID: Q14683
• Protein Accession #: Q14683
• Molecular Weight: 143 kDa (1233 aa)