SMARCA4 Antibody (OAEE00039)

Catalog No: OAEE00039

Size:100UG

Immunostaining of Brg1 by BRG-01 antibody.
A - Brg1-negative SW13 cells were transfected with Brg1-expression plasmid and analysed by Western blotting for Brg1 expression.
B - HEK293 cytoplasmic (cyt) and nuclear (nuc) lysates were analysed by Western blotting for Brg1 presence.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: BRG-01
• Isotype: IgG2a
• Host: Mouse
• Application: Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody BRG-01 recognizes an epitope within C-terminal part of human Brg1, a 205 kDa catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Griffin CT, Brennan J, Magnuson T.: The chromatin-remodeling enzyme BRG1 plays an essential role in primitive erythropoiesis and vascular development. Development. 2008 Feb;135(3):493-500.
  Trotter KW, Archer TK.: The BRG1 transcriptional coregulator. Nucl Recept Signal. 2008 Feb 1;6:e004.
  Napolitano MA, Cipollaro M, Cascino A, Melone MA, Giordano A, Galderisi U.: Brg1 chromatin remodeling factor is involved in cell growth arrest, apoptosis and senescence of rat mesenchymal stem cells. J Cell Sci. 2007 Aug 15;120(Pt 16):2904-11.
  Eroglu B, Wang G, Tu N, Sun X, Mivechi NF.: Critical role of Brg1 member of the SWI/SNF chromatin remodeling complex duringneurogenesis and neural crest induction in zebrafish.Dev Dyn. 2006 Oct;235(10):2722-35.
  Ooi L, Belyaev ND, Miyake K, Wood IC, Buckley NJ.BRG1 chromatin remodeling activity is required for efficient chromatin binding byrepressor element 1-silencing transcription factor (REST) and facilitatesREST-mediated repression.J Biol Chem. 2006 Dec 22;281(51):38974-80.

Target Info

• Gene Symbol: SMARCA4
• Gene Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Alias Symbols: ATP-dependent helicase SMARCA4;BAF190;BAF190A;brahma protein-like 1;BRG1;BRG1-associated factor 190A;BRM/SWI2-related gene 1;CSS4;global transcription activator homologous sequence;homeotic gene regulator;hSNF2b;mitotic growth and transcription activator;MRD16;nuclear protein GRB1;protein brahma homolog 1;protein BRG-1;RTPS2;SNF2;SNF2-beta;SNF2L4;SNF2LB;SNF2-like 4;sucrose nonfermenting-like 4;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4;SWI2;transcription activator BRG1.
• NCBI Gene Id: 6597
• Protein Name: Transcription activator BRG1
• Description of Target: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development, a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Binds via DLX1 to enhancers located in the intergenic region between DLX5 and DLX6 and this binding is stabilized by the long non-coding RNA (lncRNA) Evf2 (By similarity). Binds to RNA in a promiscuous manner (By similarity). Binding to RNAs including lncRNA Evf2 leads to inhibition of SMARCA4 ATPase and chromatin remodeling activities (By similarity).
• Uniprot ID: P51532
• Protein Accession #: NP_001122316.1; NP_001122317.1; NP_001122318.1; NP_001122319.1; NP_001122320.1; NP_003063.2
• Nucleotide Accession #: NM_001128844.1; NM_001128845.1; NM_001128846.1; NM_001128847.1; NM_001128848.1; NM_003072.3