SMAD4 ELISA Kit (Human) (OKEH03424)

Catalog No: OKEH03424

Size:96T

Human SMAD4 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology SMAD4 ELISA Kit (Human) (OKEH03424) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SMAD4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for SMAD4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SMAD4 captured in well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91.9%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <7.5% (n = 20)
  Mean Inter-assay CV%: <11.7% (n = 20)
• ELISA Kit Component:
  • Anti-SMAD4 Microplate: 96 Wells (12 x 8 Well strips)
  • SMAD4 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated SMAD4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 8.08 pg/mL
• Specificity: Natural and recombinant Human Mothers against decapentaplegic homolog 4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SMAD4
• Gene Full Name: SMAD family member 4
• Alias Symbols: deleted in pancreatic carcinoma locus 4;deletion target in pancreatic carcinoma 4;DPC4;JIP;MAD homolog 4;MADH4;mothers against decapentaplegic homolog 4;mothers against decapentaplegic, Drosophila, homolog of, 4;MYHRS;SMAD family member 4;SMAD, mothers against DPP homolog 4.
• NCBI Gene Id: 4089
• Protein Name: Q13485
• Description of Target: Mothers against decapentaplegic homolog 4
• Uniprot ID: https://www.uniprot.org/uniprot/Q13485
• Protein Accession #: NP_005350.1
• Nucleotide Accession #: NM_005359.5
• Protein Size (# AA): 552
• Molecular Weight: 60 kDa