SMAD3 ELISA Kit (Mouse) (OKEH03473)

Catalog No: OKEH03473

Size:96T

Mouse SMAD3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology SMAD3 ELISA Kit (Mouse) (OKEH03473) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Smad3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Smad3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Smad3 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Component:
  • Anti-Smad3 Microplate: 96 Wells (12 x 8 Well strips)
  • Smad3 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Smad3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Specificity: Natural and recombinant Mouse Mothers against decapentaplegic homolog 3
• Assay Info: Assay Methodology: Lot dependent. Can either be Quantitative or Sandwich ELISA.

Target Info

• Gene Symbol: Smad3
• Gene Full Name: SMAD family member 3
• Alias Symbols: AU022421;MAD homolog 3;mad3;Madh;Madh3;mMad3;mothers against decapentaplegic homolog 3;mothers against DPP homolog 3;Smad 3;SMAD family member 3.
• NCBI Gene Id: 17127
• Protein Name: Q8BUN5
• Description of Target: Mothers against decapentaplegic homolog 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q8BUN5
• Protein Accession #: NP_058049.3
• Nucleotide Accession #: NM_016769.4
• Protein Size (# AA): 425
• Molecular Weight: 48 kDa