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SMAD3 Antibody - middle region (OAAB09840)

400ul
$390.00
In Stock
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Predicted Species Reactivity:
Human, Mouse, Pig, Rat
Product Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Ig
Host:
Rabbit
Application:
WB, IF, IHC
Additional Information:
Precautions: SMAD3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
:::
Function: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator
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Cellular Location: Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane MAPK-mediated phosphorylation appears to have no
Reconstitution and Storage:
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Gene Symbol:
SMAD3
NCBI Gene Id:
4088
Protein Name:
Mothers against decapentaplegic homolog 3
Swissprot Id:
P84022
Protein Accession #:
NP_001138574.1
Alias Symbols:
SMAD3, MADH3, Mothers against decapentaplegic homolog 3, JV15-2, SMAD family member 3
Replacement Item:
This antibody may replace item sc-101154 from Santa Cruz Biotechnology.
Description of Target:
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Molecular Weight:
48081 Da
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SMAD3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SMAD3.
Immunogen:
This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from the Central region of human SMAD3.
Predicted Homology Based on Immunogen Sequence:
Human; Mouse; Pig; Rat
Concentration:
Approximately 0.5mg/ml. Actual concentration varies with each lot.
Datasheets/Manuals:
Printable datasheet for OAAB09840
Application Info:
WB: 1:1000
IF: 1:10~50
IHC-P: 1:10~50

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