Catalog No: OKEH03472
Size:96T
Price: $525.00
SKU
OKEH03472
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SMAD2 ELISA Kit (Mouse) (OKEH03472) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Smad2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Smad2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Smad2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Component
ComponentAmount
Anti-Smad2 Microplate96 Wells (12 x 8 Well strips)
Smad2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Smad2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
SpecificityNatural and recombinant Mouse Mothers against decapentaplegic homolog 2
Assay InfoAssay Methodology: Lot dependent. Can either be Quantitative or Sandwich ELISA.
Gene SymbolSmad2
Gene Full NameSMAD family member 2
Alias Symbols7120426M23Rik;Mad;MAD homolog 2;Madh;Madh2;Madr2;mad-related protein 2;mMad2;mothers against decapentaplegic homolog 2;mothers against DPP homolog 2;Smad 2;SMAD family member 2;Smad-2.
NCBI Gene Id17126
Protein NameQ62432
Description of TargetMothers against decapentaplegic homolog 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q62432
Protein Accession #NP_001239410.1
Nucleotide Accession #NM_001252481.1
Protein Size (# AA)467
Molecular Weight52 kDa
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