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| Datasheets/Manuals | Printable datasheet for Smad2 Antibody (Phospho-Thr220) (OAAF00207) |
|---|
| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Liquid |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
| Reconstitution and Storage | -20°C |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Smad2 around the phosphorylation site of Thr220. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity | Smad2 (Phospho-Thr220) antibody detects endogenous levels of Smad2 only when phosphorylated at threonine 220. |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Dilution | The optimal working dilution should be determined by the end user. |
| Application Info | IF: 1:100~1:500; ELISA: 1:1000 |
| Reference | Riggins G.J., Nat. Genet. 13:347-349(1996). Zhang Y., Nature 383:168-172(1996). Eppert K., Cell 86:543-552(1996) |
| Gene Symbol | SMAD2 |
|---|---|
| Gene Full Name | SMAD family member 2 |
| Alias Symbols | hMAD-2;hSMAD2;JV18;JV18-1;MAD homolog 2;MADH2;MADR2;Mad-related protein 2;mother against DPP homolog 2;mothers against decapentaplegic homolog 2;Sma- and Mad-related protein 2;SMAD family member 2;SMAD, mothers against DPP homolog 2. |
| NCBI Gene Id | 4087 |
| Protein Name | Mothers against decapentaplegic homolog 2 |
| Description of Target | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Uniprot ID | Q15796 |
| Protein Accession # | NP_001003652.1 |
| Molecular Weight | 52 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Smad2 Antibody (Phospho-Thr220) (OAAF00207)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "Smad2 Antibody (Phospho-Thr220) (OAAF00207)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Smad2 Antibody (Phospho-Thr220) (OAAF00207)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Smad2 Antibody (Phospho-Thr220) (OAAF00207)"?
This target may also be called "hMAD-2;hSMAD2;JV18;JV18-1;MAD homolog 2;MADH2;MADR2;Mad-related protein 2;mother against DPP homolog 2;mothers against decapentaplegic homolog 2;Sma- and Mad-related protein 2;SMAD family member 2;SMAD, mothers against DPP homolog 2." in publications.
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What is the shipping cost for "Smad2 Antibody (Phospho-Thr220) (OAAF00207)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Smad2 Antibody (Phospho-Thr220) (OAAF00207)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Smad2 Antibody (Phospho-Thr220) (OAAF00207)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "52 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Smad2 Antibody (Phospho-Thr220) (OAAF00207)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SMAD2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SMAD2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SMAD2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SMAD2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SMAD2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SMAD2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
