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Catalog No: OOPA00163 (Formerly GWB-8316E7)
Size:5UG
Price: $75.00
SKU
OOPA00163
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SlyD E.Coli Protein (OOPA00163)

Datasheets/ManualsPrintable datasheet for OOPA00163
Product Info
Predicted Species ReactivityEscherichia coli
Product FormatSterile Filtered colorless solution.
HostEscherichia Coli.
::Product Introduction: SlyD accessiton#: NP_755987 is a putative folding helper protein from the Escherichia coli cytosol, which has N-terminal prolyl isomerase domain of the FKBP type and a most likely unstructured C-terminal tail. SlyD is an important factor in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.
Product Description: SlyD Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 196 amino acids and having a molecular mass of 21 kDa.
Reconstitution and StorageSlyD although stable 4C for 4 weeks, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
FormulationSlyD protein solution contains 20mM Tris pH-7.5.
PurificationGreater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
PurityGreater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological ActivitySpecific activity is > 66 nmoles/min/ug, and is defined as the amount of enzyme that cleave 1umole of suc-AAFP-pNA per minute at 1C in Tris-Hcl pH8.0 using chymotrypsin.
Peptide SequenceMKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH.
Gene SymbolSlyD
Alias SymbolsFKBP-Type Peptidyl-Prolyl Cis-Trans Isomerase, SlyD.
Protein Size (# AA)Recombinant
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