SLURP1 ELISA Kit (Human) (OKCD00617)

Catalog No: OKCD00617 (Formerly GWB-KBBQS3)

Size:96WELLS

Human SLURP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	87-104%	90-97%	91-105%	94-101%
  EDTA Plasma (n=5)	78-105%	80-97%	84-95%	78-101%
  Heparin Plasma (n=5)	92-99%	99-105%	89-97%	97-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Ly6/uPAR Related Protein 1 (SLURP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Ly6/uPAR Related Protein 1 (SLURP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Ly6/uPAR Related Protein 1 (SLURP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Secreted Ly6/uPAR Related Protein 1 (SLURP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	85-101	95
  EDTA plasma(n=5)	92-101	96
  heparin plasma(n=5)	79-102	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secreted Ly6/uPAR Related Protein 1 (SLURP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secreted Ly6/uPAR Related Protein 1 (SLURP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SLURP1 Microplate: 96 Wells (12 x 8 Well Strips)
  • SLURP1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated SLURP1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, urine, saliva, tears and other biological fluids.
• Sensitivity: < 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Secreted Ly6/uPAR Related Protein 1 (SLURP1). No significant cross-reactivity or interference between Secreted Ly6/uPAR Related Protein 1 (SLURP1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SLURP1
• Gene Full Name: secreted LY6/PLAUR domain containing 1
• Alias Symbols: anti-neoplastic urinary protein;ANUP;ARS;ARS component B;ARS(component B)-81/S;ArsB;LY6LS;LY6-MT;lymphocyte antigen 6-like secreted;MDM;secreted Ly6/uPAR related protein 1;secreted Ly-6/uPAR-related protein 1;SLURP-1.
• NCBI Gene Id: 57152
• Protein Name: Secreted Ly-6/uPAR-related protein 1
• Description of Target: Has an antitumor activity (PubMed:8742060). Was found to be a marker of late differentiation of the skin. Implicated in maintaining the physiological and structural integrity of the keratinocyte layers of the skin (PubMed:14721776, PubMed:17008884). In vitro down-regulates keratinocyte proliferation; the function may involve the proposed role as modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits alpha-7-dependent nAChR currents in an allosteric manner (PubMed:14506129, PubMed:26905431). In T cells may be involved in regulation of intracellular Ca(2+) signaling (PubMed:17286989). Seems to have a immunomodulatory function in the cornea (By similarity). The function may implicate a possible role as a scavenger receptor for PLAU thereby blocking PLAU-dependent functions of PLAUR such as in cell migration and proliferation (PubMed:25168896).
• Uniprot ID: P55000
• Protein Accession #: NP_065160
• Nucleotide Accession #: NM_020427.2
• Protein Size (# AA): 103