SLPI Antibody (OAPB00538)

Catalog No: OAPB00538

Size:100UG

Western blot analysis of SLPI in Daudi cell lysate with SLPI antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry of SLPI in mouse lung tissue with SLPI antibody at 10 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. SLPI Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Positive Controls: Daudi, mouse lung tissue
• Reconstitution and Storage: SLPI antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: SLPI antibody was raised against a 17 amino acid synthetic peptide from near the center of human SLPI. The immunogen is located within amino acids 60 - 110 of SLPI.
• Purification: Affinity Purified
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-SLPI antibody is Catalog # OEPB02651
• Application Info: WB: 1-2 ug/mL
  IHC: 10 ug/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
• Reference: Abe T, Kobayashi N, Yoshimura K, et al. Expression of the secretory leukoprotease inhibitor gene in epithelial cells. J. Clin. Invest.1991; 87:2207-15.;Hiemstra PS, Fernie-King BA, McMichael J, et al. Antimicrobial peptides: mediators of innate immunity as templates for the development of novel anti-infective and immune therapies. Curr. Pharm. Des.2004; 10:2891-905.;Jana NK, Gray LR and Shugars DC. Human immunodeficiency virus type 1 stimulates the expression and production of secretory leukocyte protease inhibitor (SPLI) in oral epithelial cells: a role for SLPI in innate mucosal immunity. J. Virol.2005; 79:6432-40.;Taggart CC, Cryan S-A, Weldon S, et al. Secretory leucoprotease inhibitor binds to NF-kB binding sites in monocytes and inhibits p65 binding. J. Exp. Med.2005; 202:1659-68.
• Publications:

  MyD88 deficiency alters expression of antimicrobial factors in mouse salivary glands. PLoS One. 9, e113333 (2014). 25415419

• Description:
  View product citations for SLPI Antibody (OAPB00538) on CiteAb

Target Info

• Gene Symbol: SLPI
• Gene Full Name: secretory leukocyte peptidase inhibitor
• Alias Symbols: ALK1;ALP;antileukoproteinase;BLPI;HUSI;HUSI-1;HUSI-I;MPI;mucus proteinase inhibitor;protease inhibitor WAP4;Secretory leukocyte protease inhibitor;secretory leukocyte protease inhibitor (antileukoproteinase);seminal proteinase inhibitor;WAP four-disulfide core domain protein 4;WAP4;WFDC4.
• NCBI Gene Id: 6590
• Protein Name: Antileukoproteinase|secretory leukocyte protease inhibitor (SLPI) [Homo sapiens]
• Description of Target: Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G (PubMed:3533531, PubMed:3462719, PubMed:2039600, PubMed:2110563, PubMed:10702419, PubMed:24121345). Modulates the inflammatory and immune responses after bacterial infection, and after infection by the intracellular parasite L.major. Down-regulates responses to bacterial lipopolysaccharide (LPS) (By similarity). Plays a role in regulating the activation of NF-kappa-B and inflammatory responses (PubMed:10702419, PubMed:24352879). Has antimicrobial activity against mycobacteria, but not against salmonella. Contributes to normal resistance against infection by M.tuberculosis. Required for normal resistance to infection by L.major. Required for normal wound healing, probably by preventing tissue damage by limiting protease activity (By similarity). Together with ELANE, required for normal differentiation and proliferation of bone marrow myeloid cells (PubMed:24352879).
• Uniprot ID: P03973
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/CAA28187
• Molecular Weight: Predicted: 15 kDa Observed: 19 kDa