SLC9A1 Antibody (OABB01603)

Catalog No: OABB01603

Size:100UG

Anti-SLC9A1 antibody
All lanes: Anti SLC9A1 (OABB01603) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 91 kDa
Observed bind size: 91 kDa

Anti-SLC9A1 antibody
All lanes: Anti SLC9A1 (OABB01603) at 0.5ug/ml
WB: Recombinant Human Sodium Protein 0.5ng
Predicted bind size: 37 kDa
Observed bind size: 37 kDa

Anti-SLC9A1 antibody
IHC(P): Human Mammary Cancer Tissue

Anti-SLC9A1 antibody
IHC(P): Mouse Kidney Tissue

Anti-SLC9A1 antibody
IHC(P): Rat Kidney Tissue

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: WB: The detection limit for SLC9A1 is approximately 0.25ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  Background: NHE1, also known as SLC9A1, is an isoform of sodium–hydrogen antiporter that in humans is encoded by the SLC9A1 gene. It is mapped to 1p36.11. NHE1 is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others. The plasma membrane ion exchanger NHE1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. And a structural role for NHE1 in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. NHE1 is a plausible candidate gene for human essential hypertension.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815). Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively.
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse, Rat: By Heat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Sodium/hydrogen exchanger 1(SLC9A1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: SLC9A1
• Gene Full Name: solute carrier family 9 member A1
• Alias Symbols: APNH;LIKNS;Na(+)/H(+) antiporter, amiloride-sensitive;Na(+)/H(+) exchanger 1;Na-Li countertransporter;NHE1;NHE-1;PPP1R143;protein phosphatase 1, regulatory subunit 143;sodium/hydrogen exchanger 1;solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive);Solute carrier family 9 member 1;solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1.
• NCBI Gene Id: 6548
• Protein Name: Sodium/hydrogen exchanger 1
• Description of Target: Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
• Uniprot ID: P19634
• Molecular Weight: 90763 MW