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Catalog No: OKEH03086
Size:96T
Price: $525.00
SKU
OKEH03086
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SLC8A1 ELISA Kit (Mouse) (OKEH03086) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc8a1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Slc8a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc8a1 captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.0% (n = 20)
Mean Inter-assay CV%: <= 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Slc8a1 Microplate96 Wells (12 x 8 Well strips)
Slc8a1 Lyophilized Standard2
100X Biotinylated Slc8a1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity39.6 pg/mL
SpecificityNatural and recombinant Mouse Sodium/calcium exchanger 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSlc8a1
Gene Full Namesolute carrier family 8 (sodium/calcium exchanger), member 1
Alias SymbolsAI852629;AV344025;D930008O12Rik;Na(+)/Ca(2+)-exchange protein 1;Na/Ca exchanger;Ncx;Ncx1;sodium/calcium exchanger 1;solute carrier family 8 member 1.
NCBI Gene Id20541
Protein NameP70414
Description of TargetSodium/calcium exchanger 1
Uniprot IDhttps://www.uniprot.org/uniprot/P70414
Protein Accession #NP_035536.2
Nucleotide Accession #NM_011406.3
Protein Size (# AA)958
Molecular Weight107 kDa
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