Catalog No: OCOA18453
Size:20UG
Price: $169.00
SKU
OCOA18453
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SLC7A9 Protein Lysate (OCOA18453)

Datasheets/ManualsPrintable datasheet for SLC7A9 Protein Lysate (OCOA18453)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionSLC7A9 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolSLC7A9
Gene Full Namesolute carrier family 7 member 9
Alias Symbolsb(0,+)AT;B(0,+)-type amino acid transporter 1;BAT1;CSNU3;glycoprotein-associated amino acid transporter b0,+AT1;solute carrier family 7 (amino acid transporter light chain, bo,+ system), member 9;solute carrier family 7 (cationic amino acid transporter, y+ system), member 9;solute carrier family 7 (glycoprotein-associated amino acid transporter light chain, bo,+ system), member 9;Solute carrier family 7 member 9.
NCBI Gene Id11136
Protein NameNM_001126335, NP_001119807, P82251
Description of Targetb(0,+)-type amino acid transporter 1, B(0,+)-type amino acid transporter 1 [Homo sapiens], Homo sapiens solute carrier family 7 member 9 (SLC7A9), transcript variant 2, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P82251
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001119807
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001126335
Molecular Weight53.3 kDa
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