Catalog No: OKCD02883
Size:96WELLS
Price: $675.00
SKU
OKCD02883
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for SLC6A4 ELISA Kit (Human) (OKCD02883) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serotonin Transporter (SERT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serotonin Transporter (SERT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serotonin Transporter (SERT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Serotonin Transporter (SERT) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serotonin Transporter (SERT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serotonin Transporter (SERT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | 11.3 pg/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Serotonin Transporter (SERT). No significant cross-reactivity or interference between Serotonin Transporter (SERT) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | SLC6A4 |
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Gene Full Name | solute carrier family 6 member 4 |
Alias Symbols | 5HT transporter;5HTT;5-HTT;5-HTTLPR;5-hydroxytryptamine (serotonin) transporter;hSERT;HTT;Na+/Cl- dependent serotonin transporter;OCD1;serotonin transporter 1;SERT;SERT1;sodium-dependent serotonin transporter;solute carrier family 6 (neurotransmitter transporter), member 4;solute carrier family 6 (neurotransmitter transporter, serotonin), member 4;Solute carrier family 6 member 4. |
NCBI Gene Id | 6532 |
Protein Name | Sodium-dependent serotonin transporter |
Description of Target | Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. |
Uniprot ID | P31645 |
Protein Accession # | NP_001036.1 |
Nucleotide Accession # | NM_001045.5 |
Protein Size (# AA) | 630 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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