SLC6A4 Antibody - N-terminal region (OABB01921)

Catalog No: OABB01921

Size:100UG

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Anti-SLC6A4antibody, OABB01921, Western blotting
All lanes: Anti SLC6A4 (OABB01921) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: MM231 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 70KD
Observed bind size: 95KD

Anti-SLC6A4 antibody, OABB01921, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Anti-SLC6A4 antibody, OABB01921, IHC(P)
IHC(P): Rat Brain Tissue

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: WB: The detection limit for SLC6A4 is approximately 0.1ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  Background: SLC6A4 (Solute carrier family 6, member 4), also known as SERT (serotonin transporter), is a monoamine transporter protein. The SLC6A4 gene spans 31 kb and contains 14 exons. This protein integral membrane protein transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. This transport of serotonin by the SERT protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is the target of many antidepressant medications, including those of the SSRI class. It is a member of the sodium: neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, post-traumatic stress disorder and depression-susceptibility in people experiencing emotional trauma.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Peptide Sequence: Synthetic peptide located within the following region: DDTRHSIPATTTTLVAELHQGERETWGKK
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: By Heat
• Reference: 1. Kim, S.-J., Cox, N., Courchesne, R., Lord, C., Corsello, C., Akshoomoff, N., Guter, S., Leventhal, B. L., Courchesne, E., Cook, E. H., Jr.Transmission disequilibrium mapping at the serotonin transporter gene (SLC6A4) region in autistic disorder.Molec. Psychiat. 7: 278-288, 2002.
  2. Lesch, K.-P., Bengel, D., Heils, A., Sabol, S. Z., Greenberg, B. D., Petri, S., Benjamin, J., Muller, C. R., Hamer, D. H., Murphy, D. L.Association of anxiety-related traits with a polymorphism in the serotonin transporter gene regulatory region.Science 274: 1527-1530, 1996.
  3. Shen, S., Battersby, S., Weaver, M., Clark, E., Stephens, K., Harmar, A. J.Refined mapping of the human serotonin transporter (SLC6A4) gene within 17q11 adjacent to the CPD and NF1 genes.Europ. J. Hum. Genet. 8: 75-78, 2000.
  
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Sodium-dependent serotonin transporter(SLC6A4) detection. Tested with WB, IHC-P in Human;Rat.

Target Info

• Gene Symbol: SLC6A4
• Gene Full Name: solute carrier family 6 member 4
• Alias Symbols: 5HT transporter;5HTT;5-HTT;5-HTTLPR;5-hydroxytryptamine (serotonin) transporter;hSERT;HTT;Na+/Cl- dependent serotonin transporter;OCD1;serotonin transporter 1;SERT;SERT1;sodium-dependent serotonin transporter;solute carrier family 6 (neurotransmitter transporter), member 4;solute carrier family 6 (neurotransmitter transporter, serotonin), member 4;Solute carrier family 6 member 4.
• NCBI Gene Id: 6532
• Protein Name: Sodium-dependent serotonin transporter
• Description of Target: Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.
• Uniprot ID: P31645
• Molecular Weight: 70325 MW