SLC6A1 ELISA Kit (Human) (OKEH08356)

Catalog No: OKEH08356

Size:96T

Human SLC6A1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology SLC6A1 ELISA Kit (Human) (OKEH08356) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC6A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC6A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC6A1 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 116% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.4% (n = 20)
  Mean Inter-assay CV%: <=12.1% (n = 20)
• ELISA Kit Component:
  • SLC6A1 Microplate: 96 Wells (12 x 8 Well strips)
  • SLC6A1 Lyophilized Standard: 2
  • 100X Biotinylated SLC6A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
  Subcellular Location: Cell membrane Multi-pass membrane protein Membrane Multi-pass membrane protein Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity).
  Unigene: Hs.443874
  String: 9606.ENSP00000287766
  KEGG: hsa:6529
  MIM: 137165
  Pfam: PF00209
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 39pg/mL
• Specificity: Natural and recombinant Human Sodium- and chloride-dependent GABA transporter 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SLC6A1
• Gene Full Name: solute carrier family 6 member 1
• Alias Symbols: GABA transporter 1;GABATHG;GABATR;GAT1;MAE;sodium- and chloride-dependent GABA transporter 1;solute carrier family 6 (neurotransmitter transporter), member 1;solute carrier family 6 (neurotransmitter transporter, GABA), member 1;Solute carrier family 6 member 1.
• NCBI Gene Id: 6529
• Protein Name: P30531
• Description of Target: Sodium- and chloride-dependent GABA transporter 1
• Uniprot ID: https://www.uniprot.org/uniprot/P30531
• Protein Accession #: NP_001335179.1
• Nucleotide Accession #: NM_001348250.1