Catalog No: OKCD08659
Size:96WELLS
Price: $825.00
SKU
OKCD08659
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for SLC30A7 ELISA kit (Mouse) |
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Predicted Species Reactivity | Mouse | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colormetric|Regular detection | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 30 Member 7 (SLC30A7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 30 Member 7 (SLC30A7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 30 Member 7 (SLC30A7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Solute Carrier Family 30 Member 7 (SLC30A7) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 30 Member 7 (SLC30A7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 30 Member 7 (SLC30A7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C, -20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.053ng/mL |
Gene Symbol | SLC30A7 |
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Gene Full Name | solute carrier family 30 (zinc transporter), member 7 |
Alias Symbols | ZnT, ZnT-, ZnT7, ZnT-7, Zntl2, AI467242, 1810059J10Rik, 2610034N15Rik, 4833428C12Rik |
NCBI Gene Id | 66500 |
Protein Name | zinc transporter 7 |
Description of Target | Seems to facilitate zinc transport from the cytoplasm into the golgi apparatus. partly regulates cellular zinc homeostasis. required with znt5 for the activation of zinc-requiring enzymes, alkaline phosphatases (alps). transports zinc into the lumens of the golgi apparatus and the vesicular compartments where alps locate, thus, converting apoalps to holoalps. required with znt5 and znt6 for the activation of tnap (by similarity). |
Uniprot ID | Q9JKN1 |
Protein Accession # | NP_075703.1 |
Nucleotide Accession # | NM_023214.7 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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