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Catalog No: OKCD08659
Size:96WELLS
Price: $825.00
SKU
OKCD08659
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Datasheets/ManualsPrintable datasheet for SLC30A7 ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 30 Member 7 (SLC30A7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 30 Member 7 (SLC30A7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 30 Member 7 (SLC30A7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Solute Carrier Family 30 Member 7 (SLC30A7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 30 Member 7 (SLC30A7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 30 Member 7 (SLC30A7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
SLC30A7 Microplate96 Wells (12 x 8 Well strips)
SLC30A7 Lyophilized Standard2
100X SLC30A7 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.053ng/mL
Gene SymbolSLC30A7
Gene Full Namesolute carrier family 30 (zinc transporter), member 7
Alias SymbolsZnT, ZnT-, ZnT7, ZnT-7, Zntl2, AI467242, 1810059J10Rik, 2610034N15Rik, 4833428C12Rik
NCBI Gene Id66500
Protein Namezinc transporter 7
Description of TargetSeems to facilitate zinc transport from the cytoplasm into the golgi apparatus. partly regulates cellular zinc homeostasis. required with znt5 for the activation of zinc-requiring enzymes, alkaline phosphatases (alps). transports zinc into the lumens of the golgi apparatus and the vesicular compartments where alps locate, thus, converting apoalps to holoalps. required with znt5 and znt6 for the activation of tnap (by similarity).
Uniprot IDQ9JKN1
Protein Accession #NP_075703.1
Nucleotide Accession #NM_023214.7
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