SLC2A4 ELISA Kit (Dog) (OKEH08466)

Catalog No: OKEH08466

Size:96T

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	95-105% (n = 20)	111-121% (n = 20)	105-115% (n = 20)	100-111% (n = 20)
  EDTA plasma(n=5)	91-101% (n = 20)	91-101% (n = 20)	94-103% (n = 20)	93-102% (n = 20)
  heparin plasma(n=5)	102-111% (n = 20)	112-121% (n = 20)	105-114% (n = 20)	111-121% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology SLC2A4 ELISA Kit (Dog) (OKEH08466) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC2A4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC2A4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC2A4 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.3% (n = 20)
  Mean Inter-assay CV%: <=9.2% (n = 20)
• ELISA Kit Component:
  • SLC2A4 Microplate: 96 Wells (12 x 8 Well strips)
  • SLC2A4 Lyophilized Standard: 2
  • 100X Biotinylated SLC2A4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell.
  Subcellular Location: Cell membrane Multi-pass membrane protein Endomembrane system Multi-pass membrane protein Cytoplasm Perinuclear region Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized (By similarity). The dileucine internalization motif is critical for intracellular sequestration (By similarity). Insulin stimulation induces translocation to the cell membrane (By similarity).
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Dog Solute carrier family 2, facilitated glucose transporter member 4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SLC2A4
• Gene Full Name: solute carrier family 2 member 4
• Alias Symbols: glucose transporter type 4, insulin-responsive;GLUT4;GLUT-4;Insulin-responsive glucose transporter;solute carrier family 2, facilitated glucose transporter member 4.
• NCBI Gene Id: 403673
• Protein Name: Q9XST2
• Description of Target: Solute carrier family 2, facilitated glucose transporter member 4
• Uniprot ID: https://www.uniprot.org/uniprot/Q9XST2
• Protein Accession #: NP_001152799.1
• Nucleotide Accession #: NM_001159327.1