Catalog No: OKEH01181
Size:96T
Price: $525.00
SKU
OKEH01181
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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SLC2A3 ELISA Kit (Rat) : 96 Wells (OKEH01181)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SLC2A3 ELISA Kit (Rat) (OKEH01181) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC2A3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC2A3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC2A3 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.4% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
SLC2A3 Microplate96 Wells (12 x 8 Well strips)
SLC2A3 Lyophilized Standard2
100X Biotinylated SLC2A3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.19 ng/mL
SpecificityNatural and recombinant Rat Solute carrier family 2, facilitated glucose transporter member 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLOC100909595, Slc2a3
Gene Full Namesolute carrier family 2 member 3, solute carrier family 2, facilitated glucose transporter member 3-like
Alias Symbolsglucose transporter type 3, brain;GLUT3;GLUT-3;solute carrier family 2 (facilitated glucose transporter), member 3;Solute carrier family 2 A3 (neuron glucose transporter);solute carrier family 2, facilitated glucose transporter member 3;solute carrier family 2, facilitated glucose transporter member 3-like;solute carrier family 2, member 2.
NCBI Gene Id100909595, 25551
Protein NameQ07647
Description of TargetSolute carrier family 2, facilitated glucose transporter member 3
Uniprot IDhttps://www.uniprot.org/uniprot/Q07647
Protein Accession #NP_058798.2
Nucleotide Accession #NM_017102.2
Protein Size (# AA)493
Molecular Weight54 kDa
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