Catalog No: OKEH04280
Size:96T
Price: $525.00
SKU
OKEH04280
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SLC2A3 ELISA Kit (Human) (OKEH04280) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC2A3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC2A3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC2A3 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.0% (n = 20)
Mean Inter-assay CV%: <= 7.2% (n = 20)
ELISA Kit Component
ComponentAmount
SLC2A3 Microplate96 Wells (12 x 8 Well Strips)
SLC2A3 Standard2 x 2 ng
Sample Diluent1 x 20 mL
SLC2A3 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.098 ng/mL
SpecificityNatural and recombinant Human Solute carrier family 2, facilitated glucose transporter member 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSLC2A3
Gene Full Namesolute carrier family 2 member 3
Alias Symbolsglucose transporter type 3, brain;GLUT3;GLUT-3;solute carrier family 2 (facilitated glucose transporter), member 3;solute carrier family 2, facilitated glucose transporter member 3.
NCBI Gene Id6515
Protein NameP11169
Description of TargetSolute carrier family 2, facilitated glucose transporter member 3
Uniprot IDhttps://www.uniprot.org/uniprot/P11169
Protein Accession #NP_008862.1
Nucleotide Accession #NM_006931.2
Protein Size (# AA)496
Molecular Weight54 kDa
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