SLC2A3 ELISA Kit (Human) (OKCD07538)

Catalog No: OKCD07538

Size:96WELLS

SLC2A3 ELISA Kit (Human) (OKCD07538) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose Transporter 3 (GLUT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose Transporter 3 (GLUT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose Transporter 3 (GLUT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glucose Transporter 3 (GLUT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose Transporter 3 (GLUT3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose Transporter 3 (GLUT3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SLC2A3 Microplate: 96 Wells (12 x 8 Well strips)
  • SLC2A3 Lyophilized Standard: 2
  • 100X SLC2A3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.31ng/mL

Target Info

• Gene Symbol: SLC2A3
• Gene Full Name: solute carrier family 2 member 3
• Alias Symbols: glucose transporter type 3, brain;GLUT3;GLUT-3;solute carrier family 2 (facilitated glucose transporter), member 3;solute carrier family 2, facilitated glucose transporter member 3.
• NCBI Gene Id: 6515
• Protein Name: Solute carrier family 2, facilitated glucose transporter member 3
• Description of Target: Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane (PubMed:9477959, PubMed:26176916). Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate (PubMed:9477959, PubMed:26176916). Does not mediate fructose transport (PubMed:9477959, PubMed:26176916).
• Uniprot ID: P11169
• Protein Accession #: NP_008862.1
• Nucleotide Accession #: NM_006931.2