SLC2A3 Recombinant Antibody [14B1] (OACA12319)

Catalog No: OACA12319

Size:100UL

Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: SLC2A3 antibody at 1:1000

Overlay Peak curve showing MCF7 cells stained with OACA12319 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

IHC image of OACA12319 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of OACA12319 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of OACA12319 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Clonality: Recombinant Monoclonal
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay,
  Flow cytometry,
  Immunohistochemistry,
  Western blot
• Reconstitution and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
• Immunogen: A synthesized peptide derived from human SLC2A3
• Purification: Affinity Purified
• Concentration: Varies by lot. See vial for concentration.
• Application Info: WB: 1:500~2000
  IHC: 1:50~200
  FC: 1:50~200

Target Info

• Gene Symbol: SLC2A3
• Gene Full Name: solute carrier family 2 member 3
• Alias Symbols: glucose transporter type 3, brain;GLUT3;GLUT-3;solute carrier family 2 (facilitated glucose transporter), member 3;solute carrier family 2, facilitated glucose transporter member 3.
• NCBI Gene Id: 6515
• Protein Name: Solute carrier family 2, facilitated glucose transporter member 3
• Description of Target: Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane (PubMed:9477959, PubMed:26176916). Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate (PubMed:9477959, PubMed:26176916). Does not mediate fructose transport (PubMed:9477959, PubMed:26176916).
• Uniprot ID: P11169