Catalog No: OKEH01944
Size:96 Wells
Price: $625.00
SKU
OKEH01944
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBovine
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SLC27A5 ELISA Kit (Bovine) (OKEH01944) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-bovine SLC27A5 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SLC27A5 and incubated. The SLC27A5 found in the sample or standards competes with the biotinylated SLC27A5 for limited binding sites on the immobilized anti-bovine SLC27A5 antibody. Excess unbound biotinylated SLC27A5 and sample or standard SLC27A5 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SLC27A5 captured in the well and inversely proportional to the amount of SLC27A5 which was contained in the sample or standard.
ELISA Kit Range0.312 - 20 ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-SLC27A5 Microplate96 Wells (12 x 8 Well strips)
SLC27A5 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X SLC27A5-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity< 0.23 ng/mL
SpecificityThis assay recognizes recombinant and natural Bovine BACS. No significant cross-reactivity or interference was observed.
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Gene SymbolSLC27A5
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