Catalog No: OKEH02131
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SLC25A6 ELISA Kit (Human) (OKEH02131) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC25A6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC25A6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC25A6 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
SLC25A6 Microplate96 Wells (12 x 8 Well strips)
SLC25A6 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SLC25A6 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.045 ng/mL
SpecificityNatural and recombinant Human ADP/ATP translocase 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSLC25A6
Gene Full Namesolute carrier family 25 member 6
Alias SymbolsAAC3;adenine nucleotide translocator 3;ADP,ATP carrier protein 3;ADP,ATP carrier protein, isoform T2;ADP,ATP carrier protein, liver;ADP/ATP translocase 3;ADP/ATP translocator of liver;ANT;ANT 2;ANT 3;ANT3;ANT3Y;epididymis secretory sperm binding protein;solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6;Solute carrier family 25 member 6.
NCBI Gene Id293
Protein NameP12236
Description of TargetADP/ATP translocase 3
Uniprot ID
Protein Accession #NP_001627.2
Nucleotide Accession #NM_001636.3
Protein Size (# AA)298
Molecular Weight33 kDa
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