SLC25A6 ELISA Kit (Human) (OKEH02131)

Catalog No: OKEH02131

Size:96T

Human SLC25A6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: ELISA-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology SLC25A6 ELISA Kit (Human) (OKEH02131) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC25A6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC25A6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC25A6 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.6% (n = 20)
  Mean Inter-assay CV%: < 8.3% (n = 20)
• ELISA Kit Component:
  • SLC25A6 Microplate: 96 Wells (12 x 8 Well strips)
  • SLC25A6 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated SLC25A6 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.045 ng/mL
• Specificity: Natural and recombinant Human ADP/ATP translocase 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SLC25A6
• Gene Full Name: solute carrier family 25 member 6
• Alias Symbols: AAC3;adenine nucleotide translocator 3;ADP,ATP carrier protein 3;ADP,ATP carrier protein, isoform T2;ADP,ATP carrier protein, liver;ADP/ATP translocase 3;ADP/ATP translocator of liver;ANT;ANT 2;ANT 3;ANT3;ANT3Y;epididymis secretory sperm binding protein;solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6;Solute carrier family 25 member 6.
• NCBI Gene Id: 293
• Protein Name: P12236
• Description of Target: ADP/ATP translocase 3
• Uniprot ID: https://www.uniprot.org/uniprot/P12236
• Protein Accession #: NP_001627.2
• Nucleotide Accession #: NM_001636.3
• Protein Size (# AA): 298
• Molecular Weight: 33 kDa