Catalog No: OKEH02443
Size:96T
Price: $625.00
SKU
OKEH02443
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SLC25A4 ELISA Kit (Human) (OKEH02443) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC25A4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC25A4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC25A4 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.2% (n = 20)
Mean Inter-assay CV%: < 11.6% (n = 20)
ELISA Kit Component
ComponentAmount
SLC25A4 Microplate96 Wells (12 x 8 Well strips)
SLC25A4 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SLC25A4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.087 ng/mL
SpecificityNatural and recombinant Human ADP/ATP translocase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSLC25A4
Gene Full Namesolute carrier family 25 member 4
Alias SymbolsAAC1;Adenine nucleotide translocator 1;adenine nucleotide translocator 1 (skeletal muscle);ADP,ATP carrier protein 1;ADP,ATP carrier protein, heart/skeletal muscle;ADP,ATP carrier protein, heart/skeletal muscle isoform T1;ADP/ATP translocase 1;ANT;ANT 1;ANT1;heart/skeletal muscle ATP/ADP translocator;MTDPS12;MTDPS12A;PEO2;PEO3;PEOA2;solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4;Solute carrier family 25 member 4;T1.
NCBI Gene Id291
Protein NameP12235
Description of TargetADP/ATP translocase 1
Uniprot IDhttps://www.uniprot.org/uniprot/P12235
Protein Accession #NP_001142.2
Nucleotide Accession #NM_001151.3
Protein Size (# AA)298
Molecular Weight33 kDa
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