SLC25A37 ELISA Kit (Human) (OKCD00429)

Catalog No: OKCD00429 (Formerly GWB-KBBKZ4)

Size:96WELLS

Human SLC25A37 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitoferrin (MFRN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitoferrin (MFRN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitoferrin (MFRN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mitoferrin (MFRN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitoferrin (MFRN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitoferrin (MFRN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SLC25A37 Microplate: 96 Wells (12 x 8 Well Strips)
  • SLC25A37 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated SLC25A37 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.053 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mitoferrin (MFRN). No significant cross-reactivity or interference between Mitoferrin (MFRN) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SLC25A37
• Gene Full Name: solute carrier family 25 member 37
• Alias Symbols: HT015;MFRN;MFRN1;mitochondrial iron transporter 1;mitochondrial solute carrier protein;mitoferrin;mitoferrin-1;MSC;MSCP;predicted protein of HQ2217;PRO1278;PRO1584;PRO2217;solute carrier family 25 (mitochondrial iron transporter), member 37;Solute carrier family 25 member 37.
• NCBI Gene Id: 51312
• Protein Name: Mitoferrin-1
• Description of Target: Mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme (By similarity).
• Uniprot ID: Q9NYZ2
• Protein Accession #: NP_001304741
• Nucleotide Accession #: NM_001317812.1
• Protein Size (# AA): 338