SLC25A13 ELISA Kit (Mouse) (OKEH05862)

Catalog No: OKEH05862

Size:96T

Mouse SLC25A13 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Slc25a13 ELISA Kit (Mouse) (OKEH05862) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc25a13 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Slc25a13 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc25a13 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 98.7%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <6.8%, n = 20
  Mean Inter-Assay Precision - <9.5%, n = 20
• ELISA Kit Component:
  • SLC25A13 Microplate: 96 Wells (12 x 8 Well strips)
  • SLC25A13 Lyophilized Standard: 2
  • 100X Biotinylated SLC25A13 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.157 ng/mL
• Specificity: Natural and recombinant Mouse Calcium-binding mitochondrial carrier protein Aralar2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Slc25a13
• Gene Full Name: solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 13
• Alias Symbols: AI785475;calcium-binding mitochondrial carrier protein Aralar2;ci;citrin;Ct;Ctrn;mitochondrial aspartate glutamate carrier 2;Solute carrier family 25 member 13;solute carrier family 25, member 13.
• NCBI Gene Id: 50799
• Protein Name: Q9QXX4
• Description of Target: Calcium-binding mitochondrial carrier protein Aralar2
• Uniprot ID: https://www.uniprot.org/uniprot/Q9QXX4
• Protein Accession #: NP_001171043.1
• Nucleotide Accession #: NM_001177572.1
• Protein Size (# AA): 676