SLC23A1 ELISA Kit (Rat) (OKEH07690)

Catalog No: OKEH07690

Size:96T

Rat SLC23A1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology SLC23A1 ELISA Kit (Rat) (OKEH07690) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC23A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC23A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC23A1 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.2% (n = 20)
  Mean Inter-assay CV%: <=12.0% (n = 20)
• ELISA Kit Component:
  • SLC23A1 Microplate: 96 Wells (12 x 8 Well strips)
  • SLC23A1 Lyophilized Standard: 2
  • 100X Biotinylated SLC23A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Sodium/ascorbate cotransporter. Mediates electrogenic uptake of vitamin C, with a stoichiometry of 2 Na(+) for each ascorbate.
  Subcellular Location: Cell membrane Multi-pass membrane protein
  Unigene: Rn.144579
  String: 10116.ENSRNOP00000027048
  KEGG: rno:50621
  Pfam: PF00860
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.166ng/mL
• Specificity: Natural and recombinant Rat Solute carrier family 23 member 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SLC23A1
• Gene Full Name: solute carrier family 23 member 1
• Alias Symbols: SVCT1
• NCBI Gene Id: 50621
• Protein Name: solute carrier family 23 member 1
• Description of Target: mediates concentrative, high-affinity L-ascorbic acid transport which is driven by the Na+ electrochemical gradient [RGD, Feb 2006]
• Uniprot ID: Q9WTW7
• Protein Accession #: NP_059011.1
• Nucleotide Accession #: NM_017315.2