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Catalog No: OCOA13826
Size:20UG
Price: $179.00
SKU
OCOA13826
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SLC1A4 Protein Lysate (OCOA13826)

Datasheets/ManualsPrintable datasheet for SLC1A4 Protein Lysate (OCOA13826)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionSLC1A4 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolSLC1A4
Gene Full Namesolute carrier family 1 member 4
Alias Symbolsalanine/serine/cysteine/threonine transporter 1;ASCT1;glutamate/neutral amino acid transporter;neutral amino acid transporter A;SATT;solute carrier family 1 (glutamate/neutral amino acid transporter), member 4;Solute carrier family 1 member 4;SPATCCM.
NCBI Gene Id6509
Protein NameNM_003038, NP_003029, P43007
Description of TargetHomo sapiens solute carrier family 1 member 4 (SLC1A4), transcript variant 1, mRNA, Neutral amino acid transporter A, neutral amino acid transporter A isoform 1 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P43007
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_003029
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_003038
Molecular Weight55.7 kDa
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