Catalog No: OKEH03444
Size:96T
Price: $525.00
SKU
OKEH03444
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SLC1A3 ELISA Kit (Rat) (OKEH03444) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc1a3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Slc1a3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc1a3 captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Component
ComponentAmount
Anti-Slc1a3 Microplate96 Wells (12 x 8 Well strips)
Slc1a3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Slc1a3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.312 ng/mL
SpecificityNatural and recombinant Rat Excitatory amino acid transporter 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSlc1a3
Gene Full Namesolute carrier family 1 member 3
Alias SymbolsEAAT1;excitatory amino acid transporter 1;GLAST;GLAST-1;glial glutamate transporter;GluT-1;glutamate transporter;glutamate/aspartate transporter;sodium-dependent glutamate/aspartate transporter 1;solute carrier family 1 (glial high affinity glutamate transporter), member 3;Solute carrier family 1 member 3.
NCBI Gene Id29483
Protein NameP24942
Description of TargetExcitatory amino acid transporter 1
Uniprot IDhttps://www.uniprot.org/uniprot/P24942
Protein Accession #NP_001276870.1
Nucleotide Accession #NM_001289941.1
Protein Size (# AA)543
Molecular Weight60 kDa
Write Your Own Review
You're reviewing:SLC1A3 ELISA Kit (Rat) (OKEH03444)
Your Rating
We found other products you might like!