SLC19A1 ELISA Kit (Rat) (OKWB00391)

Catalog No: OKWB00391

Size:96T

Rat SLC19A1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	90-105%	87-104%	89-105%	89-101%
  EDTA Plasma (n=5)	86-101%	82-99%	85-101%	83-100%
  Heparin Plasma (n=5)	80-93%	81-98%	80-96%	85-97%

• ELISA Kit Principle: Aviva Systems Biology Slc19a1 ELISA Kit (Rat) (OKWB00391) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc19a1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slc19a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc19a1 captured in the well.
• ELISA Kit Range: 31.25-2000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	86-99	92
  EDTA Plasma (n=5)	89-102	98
  Heparin Plasma (n=5)	91-100	94

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Slc19a1 Microplate: 96 Wells (12 x 8 Well strips)
  • Slc19a1 Lyophilized Standard: 2 x 2 ng
  • 100X Slc19a1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 18.75 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SLC19A1
• Alias Symbols: Reduced folate carrier 1;Solute carrier family 19 member 1.
• Protein Name: Folate transporter 1
• Description of Target: Transporter for the intake of folate, reduced folates and methotrexate.
• Uniprot ID: Q62866