SLC16A1 ELISA Kit (Human) (OKCD02893)

Catalog No: OKCD02893

Size:96WELLS

Human SLC16A1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 16, Member 1 (SLC16A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 16, Member 1 (SLC16A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 16, Member 1 (SLC16A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Solute Carrier Family 16, Member 1 (SLC16A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 16, Member 1 (SLC16A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 16, Member 1 (SLC16A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SLC16A1 Microplate: 96 Wells (12 x 8 Well strips)
  • SLC16A1 Lyophilized Standard: 2
  • 100X Biotinylated SLC16A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.116 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 16, Member 1 (SLC16A1). No significant cross-reactivity or interference between Solute Carrier Family 16, Member 1 (SLC16A1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SLC16A1
• Gene Full Name: solute carrier family 16 member 1
• Alias Symbols: HHF7;MCT;MCT 1;MCT1;MCT1D;monocarboxylate transporter 1;solute carrier family 16 (monocarboxylate transporter), member 1;solute carrier family 16 (monocarboxylic acid transporters), member 1;Solute carrier family 16 member 1;solute carrier family 16, member 1 (monocarboxylic acid transporter 1).
• NCBI Gene Id: 6566
• Protein Name: Monocarboxylate transporter 1
• Description of Target: Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.
• Uniprot ID: P53985
• Protein Accession #: NP_001159968.1
• Nucleotide Accession #: NM_001166496.1
• Protein Size (# AA): 500