SLC14A1 ELISA Kit (Bovine) (OKEH07847)

Catalog No: OKEH07847

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology SLC14A1 ELISA Kit (Bovine) (OKEH07847) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC14A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC14A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC14A1 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.8% (n = 20)
  Mean Inter-assay CV%: <=9.1% (n = 20)
• ELISA Kit Component:
  • SLC14A1 Microplate: 96 Wells (12 x 8 Well strips)
  • SLC14A1 Lyophilized Standard: 2
  • 100X Biotinylated SLC14A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Urea channel that facilitates transmembrane urea transport down a concentration gradient. A constriction of the transmembrane channel functions as selectivity filter through which urea is expected to pass in dehydrated form. The rate of urea conduction is increased by hypotonic stress. Plays an important role in the kidney medulla collecting ducts, where it allows rapid equilibration between the lumen of the collecting ducts and the interstitium, and thereby prevents water loss driven by the high concentration of urea in the urine. Facilitates urea transport across erythrocyte membranes. May also play a role in transmembrane water transport, possibly by indirect means.
  Subcellular Location: Cell membrane Multi-pass membrane protein Basolateral cell membrane Multi-pass membrane protein Restricted to the basolateral membrane in various portions of the urothelium.
  Unigene: Bt.66422
  SMR: Q5QF96
  String: 9913.ENSBTAP00000044109
  KEGG: bta:493988
  Pfam: PF03253
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine Urea transporter 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SLC14A1
• Gene Full Name: solute carrier family 14 member 1
• Alias Symbols: RACH1;Solute carrier family 14 member 1;solute carrier family 14 member 1 (Kidd blood group);urea transporter 1;urea transporter B;urea transporter, erythrocyte;UT-1;UT-B.
• NCBI Gene Id: 493988
• Protein Name: Q5QF96
• Description of Target: Urea transporter 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q5QF96
• Protein Accession #: NP_001008666.1
• Nucleotide Accession #: NM_001008666.1