SLC11A2 ELISA Kit (Human) (OKCD08648)

Catalog No: OKCD08648

Size:96WELLS

SLC11A2 ELISA Kit (Human) (OKCD08648) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Divalent Metal Transporter 1 (DMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Divalent Metal Transporter 1 (DMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Divalent Metal Transporter 1 (DMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Divalent Metal Transporter 1 (DMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Divalent Metal Transporter 1 (DMT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Divalent Metal Transporter 1 (DMT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SLC11A2 Microplate: 96 Wells (12 x 8 Well strips)
  • SLC11A2 Lyophilized Standard: 2
  • 100X SLC11A2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.055ng/mL

Target Info

• Gene Symbol: SLC11A2
• Gene Full Name: solute carrier family 11 member 2
• Alias Symbols: AHMIO1;DCT1;divalent cation transporter 1;Divalent metal transporter 1;DMT1;DMT-1;natural resistance-associated macrophage protein 2;NRAMP 2;NRAMP2;solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2;solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2;Solute carrier family 11 member 2.
• NCBI Gene Id: 4891
• Protein Name: Natural resistance-associated macrophage protein 2
• Description of Target: Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution. May serve to import iron into the mitochondria.
• Uniprot ID: P49281
• Protein Accession #: NP_000608.1
• Nucleotide Accession #: NM_000617.2