Slamf7 ELISA Kit (Mouse) (OKBB01381)

Catalog No: OKBB01381

Size:96 wells

Slamf7 ELISA Kit (Mouse) (OKBB01381) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse SLAMF7 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SLAMF7 has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: S23-G224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SLAMF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse SLAMF7 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	58	226	941	61	231	967
  Standard deviation	2.32	9.72	43.29	3.11	12.94	57.05
  CV(%)	4.0	4.3	4.6	5.1	5.6	5.9

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Mouse Slamf7 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Mouse Slamf7 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Mouse Slamf7 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	58	54	47	51	52	4.03	7.7%
  Sample 2	226	265	248	245	246	13.83	5.6%
  Sample 3	941	882	888	907	904	23.00	2.5%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
  O.D.	0.045	0.111	0.169	0.280	0.471	0.805	1.403	2.142

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse SLAMF7
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S23-G224
• Description: For quantitative detection of mouse SLAMF7 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Target Info

• Gene Symbol: SLAMF7
• Gene Full Name: SLAM family member 7
• Alias Symbols: SLAM family member 7; Leukocyte cell-surface antigen; Novel Ly9; CD319; Slamf7
• NCBI Gene Id: 75345
• Protein Name: SLAM family member 7
• Description of Target: SLAM family member 7 is a protein that in humans is encoded by the SLAMF7 gene. This gene is mapped to 1; 1 H3. The surface antigen CD319 (SLAMF7) is a robust marker of normal plasma cells and malignant plasma cells in multiple myeloma. In contrast to CD138 (the traditional plasma cell marker), CD319/SLAMF7 is much more stable and allows robust isolation of malignant plasma cells from delayed or even cryopreserved samples.
• Uniprot ID: Q8BHK6
• Protein Accession #: NP_653122.2
• Nucleotide Accession #: NM_144539.5