Catalog No: OKDD00530 (Formerly GWB-KBBJB6)
Size:96 Wells
Price: $700.00
SKU
OKDD00530
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for SLAMF7 ELISA Kit (Human) (OKDD00530) |
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COA Datasheet | Click Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||||
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Application | Enzyme-linked immunosorbent assay | ||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology SLAMF7 ELISA Kit (Human) (OKDD00530) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for SLAMF7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLAMF7 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SLAMF7 captured in well. | ||||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SLAMF7 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SLAMF7 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV<10%
Inter-Assay: CV<12% | ||||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell lysates | ||||||||||||||||||||||||
Sensitivity | < 0.053 ng/mL | ||||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of SLAMF7. No significant cross-reactivity or interference between SLAMF7 and analogues was observed. | ||||||||||||||||||||||||
Assay Info | Quantitative Sandwich ELISA |
Gene Symbol | SLAMF7 |
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Gene Full Name | SLAM family member 7 |
Alias Symbols | 19A;19A24 protein;CD2 subset 1;CD2-like receptor activating cytotoxic cells;CD2-like receptor-activating cytotoxic cells;CD319;CRACC;CS1;membrane protein FOAP-12;Novel Ly9;novel LY9 (lymphocyte antigen 9) like protein;protein 19A;SLAM family member 7. |
NCBI Gene Id | 57823 |
Protein Name | SLAM family member 7 |
Description of Target | Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (PubMed:11698418). Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K (PubMed:16339536). In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (By similarity). May play a role in lymphocyte adhesion (PubMed:11802771). In LPS-activated monocytes negatively regulates production of proinflammatory cytokines (PubMed:23695528). |
Uniprot ID | Q9NQ25 |
Protein Accession # | NP_001269517.1 |
Nucleotide Accession # | NM_001282588.1 |
Protein Size (# AA) | 335 |
Molecular Weight | 37 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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