Catalog No: OKDD00530 (Formerly GWB-KBBJB6)
Size:96 Wells
Price: $700.00
SKU
OKDD00530
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Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology SLAMF7 ELISA Kit (Human) (OKDD00530) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for SLAMF7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLAMF7 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SLAMF7 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SLAMF7 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SLAMF7 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-SLAMF7 Microplate96 Wells (12 x 8 Well Strips)
SLAMF7 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X SLAMF7 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates
Sensitivity< 0.053 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of SLAMF7. No significant cross-reactivity or interference between SLAMF7 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolSLAMF7
Gene Full NameSLAM family member 7
Alias Symbols19A;19A24 protein;CD2 subset 1;CD2-like receptor activating cytotoxic cells;CD2-like receptor-activating cytotoxic cells;CD319;CRACC;CS1;membrane protein FOAP-12;Novel Ly9;novel LY9 (lymphocyte antigen 9) like protein;protein 19A;SLAM family member 7.
NCBI Gene Id57823
Protein NameSLAM family member 7
Description of TargetSelf-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (PubMed:11698418). Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K (PubMed:16339536). In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (By similarity). May play a role in lymphocyte adhesion (PubMed:11802771). In LPS-activated monocytes negatively regulates production of proinflammatory cytokines (PubMed:23695528).
Uniprot IDQ9NQ25
Protein Accession #NP_001269517.1
Nucleotide Accession #NM_001282588.1
Protein Size (# AA)335
Molecular Weight37 kDa
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