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Datasheets/Manuals | Printable datasheet for SLAMF1 Antibody (OAPB01272) (OAPB01272) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. SLAMF1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Conjugation | Unconjugated |
Application | ELISA, IF, IHC-P, WB |
Reconstitution and Storage | 2°C to 8°C |
Immunogen | SLAMF1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SLAMF1. The immunogen is located within amino acids 30 - 80 of SLAMF1. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
Concentration | 1 mg/ml |
Specificity | Two isoforms of SLAMF1 are known to exist; this antibody will recognize both isoforms. SLAMF1 antibody is predicted to not cross-react with other SLAM protein family members. |
Application Info | SLAMF1 antibody can be used for detection of SLAMF1 by Western blot at 1 - 2 ug/ml. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. (Optimal dilution should be determined by user) |
Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide. |
Reference | Cocks BG, Chang CC, Carballido JM, et al. 1995. A novel receptor involved in T cell activation. Nature 1995; 376:260-3.;Tatsuo H, Ono N, Tanaka K, et al. SLAM (CDw150) is a cellular receptor for measles virus. Nature 2000; 406:893-7.;Aversa G, Chang CC, Carballido JM, et al. Engagement of the signaling lymphocytic activation molecule (SLAM) on activated T cells results in IL-2-independent, Cyclosporin A-sensitive T cell proliferation and IFN-a production. J. Immunol. 1997; 158:4036-44.; |
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Gene Symbol | SLAMF1 |
Gene Full Name | signaling lymphocytic activation molecule family member 1 |
Alias Symbols | CD150;CDw150;IPO-3;signaling lymphocytic activation molecule;SLAM;SLAM family member 1. |
NCBI Gene Id | 6504 |
Protein Name | Signaling lymphocytic activation molecule|signaling lymphocytic activation molecule isoform b precursor [Homo sapiens] |
Description of Target | Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. SLAMF1-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAMF1 signaling seem to exist: one depending on SH2D1A (and perhaps SH2D1B) and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. Initially it has been proposed that association with SH2D1A prevents binding to inhibitory effectors including INPP5D/SHIP1 and PTPN11/SHP-2 (PubMed:11806999). However, signaling is also regulated by SH2D1A which can simultaneously interact with and recruit FYN which subsequently phosphorylates and activates SLAMF1 (PubMed:12458214). Mediates IL-2-independent proliferation of activated T-cells during immune responses and induces IFN-gamma production (By similarity). Downstreaming signaling involves INPP5D, DOK1 and DOK2 leading to inhibited IFN-gamma production in T-cells, and PRKCQ, BCL10 and NFKB1 leading to increased T-cell activation and Th2 cytokine production (By similarity). Promotes T-cell receptor-induced IL-4 secretion by CD4(+) cells (By similarity). Inhibits antigen receptor-mediated production of IFN-gamma, but not IL-2, in CD4(-)/CD8(-) T-cells (By similarity). Required for IL-4 production by germinal centers T follicular helper (T(Fh))cells (By similarity). May inhibit CD40-induced signal transduction in monocyte-derived dendritic cells (PubMed:16317102). May play a role in allergic responses and may regulate allergen-induced Th2 cytokine and Th1 cytokine secretion (By similarity). In conjunction with SLAMF6 controls the transition between positive selection and the subsequent expansion and differentiation of the thymocytic natural killer T (NKT) cell lineage. Involved in the peripheral differentiation of indifferent natural killer T (iNKT) cells toward a regulatory NKT2 type (By similarity). In macrophages involved in down-regulation of IL-12, TNF-alpha and nitric oxide in response to lipopolysaccharide (LPS) (By similarity). In B-cells activates the ERK signaling pathway independently of SH2D1A but implicating both, SYK and INPP5D, and activates Akt signaling dependent on SYK and SH2D1A (By similarity). In B-cells also activates p38 MAPK and JNK1 and JNK2 (PubMed:20231852). In conjunction with CD84/SLAMF5 and SLAMF6 may be a negative regulator of the humoral immune response (By similarity). Involved in innate immune response against Gram-negative bacteria in macrophages; probably recognizes OmpC and/or OmpF on the bacterial surface, regulates phagosome maturation and recruitment of the PI3K complex II (PI3KC3-C2) leading to accumulation of PdtIns(3)P and NOX2 activity in the phagosomes (PubMed:20818396). |
Uniprot ID | Q13291 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_003028 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SLAMF1 Antibody (OAPB01272)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "SLAMF1 Antibody (OAPB01272)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "SLAMF1 Antibody (OAPB01272)" provided in?
This item is provided in "Liquid. SLAMF1 Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SLAMF1 Antibody (OAPB01272)"?
This target may also be called "CD150;CDw150;IPO-3;signaling lymphocytic activation molecule;SLAM;SLAM family member 1." in publications.
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What is the shipping cost for "SLAMF1 Antibody (OAPB01272)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SLAMF1 Antibody (OAPB01272)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SLAMF1 Antibody (OAPB01272)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SLAMF1 Antibody (OAPB01272)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SLAMF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SLAMF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SLAMF1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SLAMF1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SLAMF1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SLAMF1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.