SLA2 ELISA Kit (Human) (OKEH08233)

Catalog No: OKEH08233

Size:96T

Human SLA2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology SLA2 ELISA Kit (Human) (OKEH08233) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLA2 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.3% (n = 20)
  Mean Inter-assay CV%: <=7.9% (n = 20)
• ELISA Kit Component:
  • SLA2 Microplate: 96 Wells (12 x 8 Well strips)
  • SLA2 Lyophilized Standard: 2
  • 100X Biotinylated SLA2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.
  Subcellular Location: Isoform 2 Cytoplasm May be cytoplasmic and is not localized to membranes.
  Unigene: Hs.713578
  SMR: Q9H6Q3
  String: 9606.ENSP00000262866
  KEGG: hsa:84174
  MIM: 606577
  Pfam: PF00017
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.053ng/mL
• Specificity: Natural and recombinant Human Src-like-adapter 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SLA2
• Gene Full Name: Src like adaptor 2
• Alias Symbols: C20orf156;MARS;modulator of antigen receptor signaling;SLAP2;SLAP-2;Src-like adapter protein 2;Src-like adapter protein-2;src-like-adapter 2.
• NCBI Gene Id: 84174
• Protein Name: Q9H6Q3
• Description of Target: Src-like-adapter 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q9H6Q3
• Protein Accession #: NP_115590.1
• Nucleotide Accession #: NM_032214.3