SIRT3 Recombinant Protein (Human) (OPED00132)

Catalog No: OPED00132

Size:500U

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Liquid. In 25mM TRIS, pH 7.5, containing 100mM NaCl, 5mM DTT, and 10% glycerol.
• Host: E. Coli
• Additional Information: Sirtuins, which are NAD-dependent lysine deacetylases, have been implicated in the control of longevity in yeast and C. elegans and, in yeast, have been shown to be necessary for the longevity increase effected by caloric restriction. Human SIRT3, one of seven human sirtuins, has been assigned, along with SIRT1 and SIRT2, to the class I sirtuin homology group.
• Reconstitution and Storage: Store at -80C
• Purification: Partially purified by single-step affinity chromatography and gel filtration.
• Reference: SIRT3 Deacetylates Ceramide Synthases: Implications for Mitochondrial Dysfunction and Brain Injury: S.A. Novgorodov, et al.; J. Biol. Chem. 291, 1957 (2016), Application(s): Deacetylation assay with mouse brain cells, Abstract; Full Text

Target Info

• Gene Symbol: SIRT3
• Gene Full Name: sirtuin 3
• Alias Symbols: mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase;NAD-dependent deacetylase sirtuin-3, mitochondrial;NAD-dependent protein deacetylase sirtuin-3, mitochondrial;regulatory protein SIR2 homolog 3;silent mating type information regulation 2, S.cerevisiae, homolog 3;SIR2L3;sir2-like 3;SIR2-like protein 3;sirtuin type 3.
• NCBI Gene Id: 23410
• Protein Name: NAD-dependent protein deacetylase sirtuin-3, mitochondrial
• Description of Target: NAD-dependent protein deacetylase (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:23283301, PubMed:24121500, PubMed:24252090, PubMed:19535340). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:23283301, PubMed:24121500, PubMed:24252090). Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O (PubMed:16788062, PubMed:18680753, PubMed:24121500, PubMed:24252090, PubMed:19535340). Contributes to the regulation of the cellular energy metabolism (PubMed:24252090). Important for regulating tissue-specific ATP levels (PubMed:18794531). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed:23283301).
• Uniprot ID: Q9NTG7
• Protein Accession #: NP_776770.2
• Nucleotide Accession #: NM_174345.4
• Protein Size (# AA): Recombinant
• Molecular Weight: 33 kDa