SIRT3 ELISA Kit (Human) (OKEH03460)

Catalog No: OKEH03460

Size:96T

Human SIRT3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology SIRT3 ELISA Kit (Human) (OKEH03460) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SIRT3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for SIRT3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SIRT3 captured in well.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90.9%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.9% (n = 20)
  Mean Inter-assay CV%: <= 11.6% (n = 20)
• ELISA Kit Component:
  • Anti-SIRT3 Microplate: 96 Wells (12 x 8 Well strips)
  • SIRT3 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated SIRT3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.156 ng/mL
• Specificity: Natural and recombinant Human NAD-dependent deacetylase sirtuin-3, mitochondrial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SIRT3
• Gene Full Name: sirtuin 3
• Alias Symbols: mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase;NAD-dependent deacetylase sirtuin-3, mitochondrial;NAD-dependent protein deacetylase sirtuin-3, mitochondrial;regulatory protein SIR2 homolog 3;silent mating type information regulation 2, S.cerevisiae, homolog 3;SIR2L3;sir2-like 3;SIR2-like protein 3;sirtuin type 3.
• NCBI Gene Id: 23410
• Protein Name: Q9NTG7
• Description of Target: NAD-dependent protein deacetylase sirtuin-3, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/Q9NTG7
• Protein Accession #: NP_001017524.1
• Nucleotide Accession #: NM_001017524.2
• Protein Size (# AA): 399
• Molecular Weight: 44 kDa