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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for anti-SIRT1 (OAAJ02761) antibody |
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Predicted Species Reactivity | Human, Mouse |
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Product Format | Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | WB |
Additional Information | Subcellular Location: Cytoplasm. Mitochondrion and Nucleus, PML body. Cytoplasm. Nucleus. Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. Shuttles between nucleus and cytoplasm. Colocalizes in the nucleus with XBP1 isoform 2. Tissue Specificity: Widely expressed. Similarity: Belongs to the sirtuin family. Class I subfamily. |
Reconstitution and Storage | Stable at -20°C for at least 1 year. |
Immunogen | A synthesized peptide derived from human SirT1 around the phosphorylation site of Serine 47 |
Purification | The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns. |
Concentration | 1 mg/ml |
Specificity | Phospho-SirT1 (Ser47) antibody detects endogenous levels of SirT1 only when phosphorylated at Serine 47. |
Application Info | WB: 1:500-2,000 Important Note: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween20 at 4°C with gentle shaking, overnight. Optimal dilutions should be determined by the end user. |
Gene Symbol | SIRT1 |
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Gene Full Name | sirtuin 1 |
Alias Symbols | SIR2, SIR2L1, SIR2alpha |
NCBI Gene Id | 23411 |
Description of Target | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. |
Uniprot ID | Q96EB6 |
Molecular Weight | 110 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SIRT1 Antibody (Phospho-Ser47) (OAAJ02761)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
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How long will it take to receive "SIRT1 Antibody (Phospho-Ser47) (OAAJ02761)"?
This item is available "Domestic: within 2-3 week delivery | International: 2-3 weeks".
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What buffer format is "SIRT1 Antibody (Phospho-Ser47) (OAAJ02761)" provided in?
This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SIRT1 Antibody (Phospho-Ser47) (OAAJ02761)"?
This target may also be called "SIR2, SIR2L1, SIR2alpha" in publications.
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What is the shipping cost for "SIRT1 Antibody (Phospho-Ser47) (OAAJ02761)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SIRT1 Antibody (Phospho-Ser47) (OAAJ02761)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SIRT1 Antibody (Phospho-Ser47) (OAAJ02761)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "110 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SIRT1 Antibody (Phospho-Ser47) (OAAJ02761)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SIRT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SIRT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SIRT1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SIRT1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SIRT1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SIRT1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.